Kentucky (36-17) hit four home runs. Brianna Pratt (18-5) took the loss for Miami (39-16-1).
Holly Blaska and Maggie Murphy had hits for the Mid-American Conference champion RedHawks.
Miami was scheduled to face St. Francis (Pa.) Saturday night in an elmination game. A win would move the RedHawks into another elmination game later Saturday vs. Virginia Tech or Kentucky.
The final day of the double-elimination regional is set for Sunday.