Rubio's Tampa staff was asked to leave after the building owner said rallies had become too disruptive to the other tenants.
"Just as we are doing in Tampa, our staff in Jacksonville will continue to provide services to Northeast Florida, despite not having an office, until a new office space is secured," Mandreucci said.
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"Those who disagree with President Trump and Senator Rubio certainly have a right to exercise their First Amendment rights, and most of them have done so in a productive and respectful way. But, unfortunately, some of them have chosen to do so in a manner that potentially hinders their fellow Floridians' ability to receive help from our office," she added.
The existing lease in Jacksonville at 1650 Prudential Drive on the Southbank, is currently month-to-month, which requires a 30-day notice to terminate.
The current lease ends on April 30 and Rubio staff will start looking for new office space this week.